Anti-TRAP (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P29965
Gene Id	959
Gene Symbols	CD40LG
Protein Name / Synonyms	TRAP (N-term)
Clonality	Polyclonal
Preparation	This TRAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-62 amino acids from the N-terminal region of human TRAP.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IF, IHC-P, FC
Research Area	Inflammation, Ras/Rap Signaling
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

TRAP is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in its gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome.

Specificity

Predicted Reactivity

Bovine, Pig

Other Names

CD40LG; CD40L; TNFSF5; TRAP; CD40 ligand; T-cell antigen Gp39; TNF-related activation protein; Tumor necrosis factor ligand superfamily member 5; CD_antigen=CD154; CD40 ligand, membrane form; CD40 ligand, soluble form

NCBI Accession #

NP_000065.1

Other Accession #

Q95MQ5; P51749

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

29274

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; IF: 1:10-50; FC: 1:10-50

	Western blot analysis of TRAP antibody (N-term) (Cat# 102-16815) in NCI-H460 cell line lysates (35ug/lane). TRAP (arrow) was detected using the purified Pab.
	TRAP Antibody (N-term) (Cat# 102-16815) IHC analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TRAP Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
	Confocal immunofluorescent analysis of TRAP Antibody (N-term)(Cat# 102-16815) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
	TRAP antibody (N-term) (Cat# 102-16815) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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