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Anti-Trefoil factor 3 (TFF3) Antibody, Biotinylated
Catalog #: 130-10147B-50
Rabbit anti-Trefoil factor 3 (TFF3) Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q07654 |
| Gene Id | 7033 |
| Gene Symbols | TFF3 ITF TFI |
| Clonality | Polyclonal |
| Immunogen | The immunogen was a recombinant protein. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum followed by Protein A/G affinity chromatography. |
| Isotype | IgG |
| Recommended Applications | ELISA (recommended work dilution= 1: 8, 000), Western Blotting (recommended work dilution= 1:1000-5, 000) , (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant protein Inhibin alpha. The membrane blot was probed with anti- Inhibin alpha primary antibody (0.2 µg/ml). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate recombinant protein Inhibin alpha from E.coli cell (14kDa). |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Trefoil factor 3 (TFF3) is a protein that has a trefoil motif characterized. Though those functions are not defined, they may protect the mucosa from insults. It can be used as a marker to improve the accuracy and acceptability of the detection/screening of Barrett's Oesophagus.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
This antibody binds to its immunogen, and did not show any cross reactivity with unrelated antigens in ELISA. Cross reactivity with mouse and rat Trefoil factor 3 has not been tested yet.
Related Products
Mouse Anti-Human Trefoil factor 3 (Cat: DS-MB-03361) ; Trefoil factor 3, recombinant human protein, E.coli derived (Cat: 230-00228)
References
- Daniel K. Podolsky, et al. Colitis-associated variant of TLR2 causes impaired mucosal repair due to TFF3 deficiencyGastroenterology. 2009 July; 137(1): 209220.
- P. L. Beck, et al. Phylogenomic Exploring the interplay of barrier function and leukocyte recruitment in intestinal inflammation by targeting fucosyltransferase VII and trefoil factor 3. Am J Physiol Gastrointest Liver Physiol. 2010 July; 299(1): G43G53.
Expiration:
12 months from the date of shipment when stored properly.