Anti-TRIM28 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q13263
Gene Id	10155
Gene Symbols	TRIM28
Protein Name / Synonyms	TRIM28 (N-term)
Clonality	Polyclonal
Preparation	This TRIM28 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 178-207 amino acids from the N-terminal region of human TRIM28.
Concentration (lot specific)	0.470 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.

Specificity

Other Names

TRIM28; KAP1; RNF96; TIF1B; Transcription intermediary factor 1-beta; E3 SUMO-protein ligase TRIM28; KRAB-associated protein 1; KRAB-interacting protein 1; Nuclear corepressor KAP-1; RING finger protein 96; Tripartite motif-containing protein 28

NCBI Accession #

NP_005753.1

Other Accession #

NP_005753.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

88550

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; IF: 1:10-50

	TRIM28 Antibody (N-term) (Cat# 102-11288) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the TRIM28 antibody detected the TRIM28 protein (arrow).
	TRIM28 Antibody (N-term) (Cat# 102-11288)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TRIM28 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
	Fluorescent confocal image of Hela cell stained with TRIM28 Antibody (N-term)(Cat#RB09760).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TRIM28 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). TRIM28 immunoreactivity is localized to Nucleus significantly.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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