Anti-TRIM32 Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Rat
Host Species	Rabbit
Gene Id	22954
Protein Name / Synonyms	TRIM32
Concentration (lot specific)	0.74 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

BBS11 antibody, HT2A antibody, LGMD2H antibody, TATIP antibody, TRIM32 antibody, zinc-finger protein HT2A antibody, 72 kDa Tat-interacting protein antibody, tripartite motif-containing 32 antibody, tripartite motif-containing protein 32 antibody, zinc finger protein HT2A antibody, TAT-interactive protein, 72-KD antibody, E3 ubiquitin-protein ligase TRIM32 antibody, tripartite motif containing 32 antibody

Specificity

Species Reactivity

Human, Rat

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

72 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 289 of TRIM32 (Uniprot ID# Q13049)

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

TRIM32 antibody detects TRIM32 protein at cytoplasm by immunofluorescent analysis.
Sample: A375 cells were fixed in ice-cold MeOH for 5 min.
Green: TRIM32 protein stained by TRIM32 antibody (107-11099) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 ?m.

TRIM32 antibody detects TRIM32 protein by western blot analysis.
A. 30 ?g 293T whole cell lysate/extract
7.5 % SDS-PAGE
TRIM32 antibody (107-11099) dilution: 1:1000

TRIM32 antibody detects TRIM32 protein by western blot analysis.
A. 5 ?g 293T whole cell lysate/extract
B. 5 ?g whole cell lysate/extract of DDDDK-human TRIM32-transfected 293T cells
7.5 % SDS-PAGE
TRIM32 antibody (107-11099) dilution: 1:20000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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