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Anti-Triosephosphate isomerase (C-term) Antibody
Catalog #: 107-10463
Triosephosphate isomerase antibody [C2C3], C-Terminus, 1 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Gene Id | 7167 |
| Protein Name / Synonyms | Triosephosphate isomerase, C-term |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
MGC88108 antibody, TPI antibody, TPI1 antibody, triose-phosphate isomerase antibody, triosephosphate isomerase antibody, triosephosphate isomerase 1 antibody
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
31 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 187 and 249 of Triosephosphate isomerase (Uniprot ID# P60174)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Triosephosphate isomerase antibody [C2C3], C-term detects Triosephosphate isomerase protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Triosephosphate isomerase protein stained by Triosephosphate isomerase antibody [C2C3], C-term (107-10463) diluted at 1:500.
Blue: Hoechst 33342 staining.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Triosephosphate isomerase protein stained by Triosephosphate isomerase antibody [C2C3], C-term (107-10463) diluted at 1:500.
Blue: Hoechst 33342 staining.
Triosephosphate isomerase antibody [C2C3], C-term detects TPI1 protein by Western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg GL261 whole cell lysate/extract
C. 30 µg C8D30 whole cell lysate/extract
D. 30 µg NIH-3T3 whole cell lysate/extract
E. 30 µg BCL-1 whole cell lysate/extract
F. 30 µg Raw264.7 whole cell lysate/extract
G. 30 µg C2C12 whole cell lysate/extract
12 % SDS-PAGE
Triosephosphate isomerase antibody [C2C3], C-term (107-10463) dilution: 1:1000
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg GL261 whole cell lysate/extract
C. 30 µg C8D30 whole cell lysate/extract
D. 30 µg NIH-3T3 whole cell lysate/extract
E. 30 µg BCL-1 whole cell lysate/extract
F. 30 µg Raw264.7 whole cell lysate/extract
G. 30 µg C2C12 whole cell lysate/extract
12 % SDS-PAGE
Triosephosphate isomerase antibody [C2C3], C-term (107-10463) dilution: 1:1000
Triosephosphate isomerase antibody [C2C3], C-term detects TPI1 protein by Western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat2 whole cell lysate/extract
12 % SDS-PAGE
Triosephosphate isomerase antibody [C2C3], C-term (107-10463) dilution: 1:1000
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat2 whole cell lysate/extract
12 % SDS-PAGE
Triosephosphate isomerase antibody [C2C3], C-term (107-10463) dilution: 1:1000
Immunohistochemical analysis of paraffin-embedded SNU-16 xenograft, using TPI1 (107-10463) antibody at 1:100 dilution.
Sample(30 µg of whole cell lysate)
A:A431
B:H1299
12% SDS PAGE
107-10463 diluted at 1:2000
A:A431
B:H1299
12% SDS PAGE
107-10463 diluted at 1:2000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.