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Anti-Troponin Antibody
Catalog #: 130-10087
Rabbit Anti-Human Troponin Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | P45379 |
| Gene Id | 7139 |
| Gene Symbols | TNNT2 |
| Clonality | Polyclonal |
| Immunogen | Immunogen was recombinant protein from human troponin. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum followed by Protein A/G affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution= 1: 80, 000) |
| Antigen Catalog Number | 230-00048 |
| Reconstitution | Product is supplied as a powder obtained from lyophilization of purified antibody in PBS without preservatives. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml. |
| Research Area | Cardiovascular Disease |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Troponin is a complex of three regulatory proteins (troponin C, troponin I and troponin T) that is integral to muscle contraction in skeletal and cardiac muscle, but not smooth muscle. Troponin is a component of thin filaments (along with actin and tropomyosin), and is the protein to which calcium binds to accomplish this regulation. Troponin has three subunits, TnC, TnI, and TnT. When calcium is bound to specific sites on TnC, tropomyosin rolls out of the way of the actin filament active sites, so that myosin (a molecular motor organized in muscle thick filaments) can attach to the thin filament and produce force and/or movement.
Specificity
The antibody binds to human troponin. The specificity for binding to recombinant protein, cellular protein and native antigen is not defined. Cross reactivity with mouse or rat troponin was not tested.
Storage
Store at 4°C if intended for use within one month, otherwise, store at -20°C to -80°C. The lyophilized antibody is stable for at least 18 months after the date of receipt when stored at -20°C to -80°C. After reconstitution, it can be aliquoted and stored frozen at -20°C to -80°C in a manual defrost freezer for at least 6 months without detectable loss of activity. Upon reconstitution, the antibody can also be stored for 1 month at 4°C. Please avoid freeze-thaw cycles, as this will lower the activity of the antibody.
Related Products
Mouse Anti-Human Troponin C (cat# DS-MB-03430) Goat Anti-Human Troponin I (Cardiac) (cat#DS-PB-02013)
References
- PDB 1J1E; et al. (2003). "Structure of the core domain of human cardiac troponin in the Ca (2+)-saturated form". Nature 424 (6944): 3541.
- Moses, Marsha A. et al. (1999). "Troponin I is present in human cartilage and inhibits angiogenesis". Proceedings of the National Academy of Sciences of the United States of America 96 (6): 26452650.
Expiration:
12 months from the date of shipment when stored properly.