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Anti-Troponin Antibody, Biotinylated
Catalog #: 130-10087B-50
Rabbit anti-Troponin Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P45379 |
| Gene Id | 7139 |
| Gene Symbols | TNNT2 |
| Clonality | Polyclonal |
| Immunogen | Immunogen was recombinant protein from human troponin. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum followed by Protein A/G affinity chromatography. |
| Isotype | IgG |
| Recommended Applications | ELISA (recommended work dilution= 1: 80, 000) |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Research Area | Cardiovascular Disease |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Troponin is a complex of three regulatory proteins (troponin C, troponin I and troponin T) that is integral to muscle contraction in skeletal and cardiac muscle, but not smooth muscle. Troponin is a component of thin filaments (along with actin and tropomyosin), and is the protein to which calcium binds to accomplish this regulation. Troponin has three subunits, TnC, TnI, and TnT. When calcium is bound to specific sites on TnC, tropomyosin rolls out of the way of the actin filament active sites, so that myosin (a molecular motor organized in muscle thick filaments) can attach to the thin filament and produce force and/or movement.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
The antibody binds to human troponin. The specificity for binding to recombinant protein, cellular protein and native antigen is not defined. Cross reactivity with mouse or rat troponin was not tested.
Related Products
Mouse Anti-Human Troponin C (cat# DS-MB-03430) Goat Anti-Human Troponin I (Cardiac) (cat#DS-PB-02013)
References
- PDB 1J1E; et al. (2003). "Structure of the core domain of human cardiac troponin in the Ca (2+)-saturated form". Nature 424 (6944): 3541.
- Moses, Marsha A. et al. (1999). "Troponin I is present in human cartilage and inhibits angiogenesis". Proceedings of the National Academy of Sciences of the United States of America 96 (6): 26452650.
Expiration:
12 months from the date of shipment when stored properly.