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Anti-TSG101 Antibody
Catalog #: 144-61333
TSG101 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q99816 |
| Gene Id | 7251 |
| Gene Symbols | TSG101 |
| Protein Name / Synonyms | TSG101, TSG10, VPS23, tumor susceptibility 101 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IP |
| Research Area | Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression.
Dilutions
WB 1:200 - 1:2000
IP 1:20 - 1:50
IP 1:20 - 1:50
Format
Calculated MW
31kDa/43kDa
Observed MW
48kDa
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human TSG101 (NP_006283.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using TSG101 antibody (144-61333) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Immunoprecipitation analysis of 150ug extracts of HeLa cells using 3ug TSG101 antibody (144-61333). Western blot was performed from the immunoprecipitate using TSG101 antibody (144-61333) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.