Anti-TSH2 (N-term) Antibody

Product Description

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q9NRE2
Gene Id	128553
Gene Symbols	TSHZ2
Protein Name / Synonyms	TSH2 (N-term)
Clonality	Polyclonal
Preparation	This TSH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 232-261 amino acids from the N-terminal region of human TSH2.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC, IF
Shipping Type	Blue ice
Storage	-20°C

TSH2 is transcriptional regulator involved in developmental processes (Potential).

Predicted Reactivity

Mouse, Pig

Other Names

TSHZ2; C20orf17; TSH2; ZNF218; Teashirt homolog 2; Ovarian cancer-related protein 10-2; Zinc finger protein 218

NCBI Accession #

NP_001180350.1;NP_775756.3

Other Accession #

A5GFT6; Q68FE9

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

115005

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50; IF: 1:10-50

	Western blot analysis of TSH2 Antibody (N-term) (Cat# 102-16461) in CEM cell line lysates (35ug/lane).TSH2 (arrow) was detected using the purified Pab.
	TSH2 Antibody (N-term) (Cat# 102-16461) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TSH2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
	TSH2 Antibody (N-term) (Cat# 102-16461) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Fluorescent confocal image of U251 cell stained with TSH2 Antibody (N-term)(Cat# 102-16461).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TSH2 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). TSH2 immunoreactivity is localized to Nucleus and Vesicles significantly.

HUMAN

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

Product Description