Synonyms
MGC19578 antibody, RDS antibody, TST antibody, thiosulfate sulfurtransferase antibody, thiosulfate sulfurtransferase (rhodanese) antibody
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
33 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 270 of TST (Uniprot ID# Q16762)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using TST(107-10596) antibody at 1:250 dilution.

TST antibody detects TST protein at mitochondria on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
TST antibody (107-10596) dilution: 1:500.

Sample (50 ug of whole cell lysate)
A: Mouse liver
12% SDS PAGE
107-10596 diluted at 1:1000

TST antibody detects TST protein by Western blot analysis.
A. 50 µg rat liver lysate/extract
12 % SDS-PAGE
TST antibody (107-10596) dilution: 1:1000

Sample (30 ug of whole cell lysate)
A: Hep G2
12% SDS PAGE
107-10596 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.