TTC11 antibody, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Gene Id | 51024 |
| Protein Name / Synonyms | TTC11 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
TTC11 antibody, FIS1 antibody, TPR repeat protein 11 antibody, hFis1 antibody, tetratricopeptide repeat domain 11 antibody, mitochondrial fission 1 protein antibody, FIS1 homolog antibody, H_NH0132A01.6 antibody, tetratricopeptide repeat protein 11 antibody, fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae) antibody
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
17 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 152 of TTC11 (Uniprot ID# Q9Y3D6)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
TTC11 antibody detects TTC11 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37? for 30 min.
Green: TTC11 protein stained by TTC11 antibody (107-10944) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37? for 30 min.
Green: TTC11 protein stained by TTC11 antibody (107-10944) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Sample (50 ug of whole cell lysate)
A: mouse brain
15% SDS PAGE
107-10944 diluted at 1:5000
A: mouse brain
15% SDS PAGE
107-10944 diluted at 1:5000
TTC11 antibody detects FIS1 protein by Western blot analysis.
A. 50 µg rat brain lysate/extract
15 % SDS-PAGE
TTC11 antibody (107-10944) dilution: 1:1000
A. 50 µg rat brain lysate/extract
15 % SDS-PAGE
TTC11 antibody (107-10944) dilution: 1:1000
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using FIS1(107-10944) antibody at 1:100 dilution.
Sample (30 ug of whole cell lysate)
A: Raji
15% SDS PAGE
107-10944 diluted at 1:10000
A: Raji
15% SDS PAGE
107-10944 diluted at 1:10000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.