Synonyms
FLJ13946 antibody, FLJ77601 antibody, TTC30A antibody, tetratricopeptide repeat protein 30A antibody, TPR repeat protein 30A antibody, tetratricopeptide repeat domain 30A antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
76 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 347 and 665 of TTC30A (Uniprot ID# Q86WT1)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using TTC30A(107-10683) antibody at 1:100 dilution.

Sample(30 ug whole cell lysate)
A:A431
7.5% SDS PAGE
107-10683 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.