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Anti-Tubulin alpha Antibody
Catalog #: DS-MB-02478
Rat Anti-Tubulin alpha
Product Description
Specifications
| Size | 0.5 mg |
| Estimated Lead Time | 1-2 weeks |
| Species | Yeast |
| Specificity | This product recognizes the alpha subunit of tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin), see Wehland, J. et al. for details. The epitope recognized by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Glu-Glu-Tyr in Tyr-Tubulin). The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin, see Burns, R. for details. This product is routinely tested in ELISA on Tubulin. |
| Protein Name / Synonyms | Tubulin alpha |
| Concentration (lot specific) | IgG concentration 1.0 mg/ml |
| Recommended Applications | Immunohistology - Frozen, ELISA (dilution: 1/100, 1/1000), Immunofluorescence, Immunoprecipitation, Radioimmunoassays, Western Blotting |
| Research Area | Cytoskeletal Reorganization |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
This product is suitable for use as a loading control.
Storage/Stability
Store at +4 °C or at -20 °C if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Format
Supplied as:
Purified IgG - liquid
Formulation:
Phosphate buffered saline
Format Type:
Purified
Preparation
Immunogen:
Yeast tubulin
Preservative:
0.09% Sodium Azide
This product
Spleen cells from immunised LOU rats were fused with cells of the Y3.Ag.1.2.3 rat myeloma cell line.
Expiration:
12 months from the date of shipment when stored properly.