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Anti-TUFM (N-term) Antibody

Catalog #: 102-15226

Rabbit Anti-Human TUFM (N-term) Antibody, 400 ul

Product Description

Specifications

Size400 µL
Estimated Lead Time1-2 weeks
SpeciesHuman
Host SpeciesRabbit
Accession Number
P49411
Gene Id
7284
Gene Symbols
TUFM
Protein Name / Synonyms
TUFM (N-term)
ClonalityPolyclonal
PreparationThis TUFM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 75-104 amino acids from the N-terminal region of human TUFM.
Concentration (lot specific)
2.0 mg/ml
Recommended ApplicationsWestern Blotting, IHC-P, FC
Shipping TypeBlue ice
Storage-20°C

Description

Introduction

TUFM is a protein which participates in protein translation in mitochondria. This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

Specificity

Predicted Reactivity
Mouse, Rat, Bovine
Other Names
TUFM; Elongation factor Tu, mitochondrial; P43
NCBI Accession #
NP_003312.3
Other Accession #
P85834; Q8BFR5; P49410

Format

Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
49542
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50
Western blot analysis of TUFM Antibody (N-term) (Cat# 102-15226) in Ramos, Hela cell line lysates (35ug/lane). TUFM (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with TUFM Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
TUFM Antibody (N-term) (Cat# 102-15226) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of TUFM (arrow) using rabbit polyclonal TUFM Antibody (N-term) (Cat# 102-15226). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TUFM gene.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.