Anti-TWIST1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q15672
Gene Id	7291
Gene Symbols	TWIST1
Protein Name / Synonyms	TWIST1, ACS3, BPES2, BPES3, CRS, CRS1, CSO, SCS, TWIST, bHLHa38, twist-related protein 1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Research Area	Angiogenesis, Cardiovascular Disease, Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Background

Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue; in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW

20kDa

Observed MW

21kDa

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TWIST1 (NP_000465.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of U-87MG cells, using TWIST1 antibody (144-61361) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Western blot - TWIST1 antibody (144-61361)

Immunohistochemistry of paraffin-embedded human tonsil using TWIST1 antibody (144-61361) at dilution of 1:100 (40x lens).
Immunohistochemistry - TWIST1 antibody (144-61361)

Immunohistochemistry of paraffin-embedded human lung cancer using TWIST1 antibody (144-61361) at dilution of 1:100 (40x lens).
Immunohistochemistry - TWIST1 antibody (144-61361)

Expiration:

12 months from the date of shipment when stored properly.

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