Anti-TYRO10 Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q16832
Gene Id	4921
Gene Symbols	DDR2
Protein Name / Synonyms	TYRO10
Clonality	Polyclonal
Preparation	This TYRO10 antibody is generated from rabbits immunized with a his tag recombinant protein of human TYRO10.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Receptor tyrosine kinases (RTKs) play a key role in the communication of cells with their microenvironment. These molecules are involved in the regulation of cell growth, differentiation and metabolism. In several cases the biochemical mechanism by which RTKs transduce signals across the membrane has been shown to be ligand induced receptor oligomerization and subsequent intracellular phosphorylation. This autophosphorylation leads to phosphorylation of cytosolic targets as well as association with other molecules, which are involved in pleiotropic effects of signal transduction. RTKs have a tripartite structure with extracellular, transmembrane and cytoplasmic regions. There are several subclasses of RTKs and TYRO10 belongs to a novel subclass. The deduced amino acid sequence of TYRO10 has a unique extracellular region encompassing a factor VIII-like domain, not previously described for RTKs.

Specificity

Other Names

DDR2; NTRKR3; TKT; TYRO10; Discoidin domain-containing receptor 2; CD167 antigen-like family member B; Discoidin domain-containing receptor tyrosine kinase 2; Neurotrophic tyrosine kinase, receptor-related 3; Receptor protein-tyrosine kinase TKT; Tyrosine-protein kinase TYRO10; CD_antigen=CD167b; Flags: Precursor

NCBI Accession #

NP_001014796.1;NP_006173.2

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

96736

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; FC: 1:10-50

	Western blot analysis of TYRO10 (arrow) using rabbit polyclonal TYRO10 Antibody (Cat# 102-17562). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DDR2 gene (Lane 2) (Origene Technologies).
	Western blot analysis of TYRO10 Antibody (Cat# 102-17562) in Jurkat(lane 1), 293(lane 2) and Hela(lane 3) cell line lysates (35ug/lane). TYRO10 (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human normal lung with TYRO10 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Flow cytometric analysis of jurkat cells using TYRO10 Antibody(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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