Rabbit Anti-Human Tyrosinase (C-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P14679 |
| Gene Id | 7299 |
| Gene Symbols | TYR |
| Protein Name / Synonyms | Tyrosinase (C-term) |
| Clonality | Polyclonal |
| Preparation | This Tyrosinase antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 486-513 amino acids from the C-terminal region of human Tyrosinase. |
| Concentration (lot specific) | 2.000 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, IF, FC |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
TYR catalyzes the first 2 steps, and at least 1 subsequent step, in the conversion of tyrosine to melanin. The protein has both tyrosine hydroxylase and dopa oxidase catalytic activities, and requires copper for function. Mutations in this protein result in oculocutaneous albinism, and nonpathologic polymorphisms result in skin pigmentation variation.
Specificity
Other Names
TYR; Tyrosinase; LB24-AB; Monophenol monooxygenase; SK29-AB; Tumor rejection antigen AB
NCBI Accession #
NP_000363.1
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
60393
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50; IF: 1:10-50; FC: 1:10-50
 | Western blot analysis of Tyrosinase antibody (C-term) (Cat# 102-17294) in HepG2 cell line lysates (35ug/lane). Tyrosinase (arrow) was detected using the purified Pab. |
 | Tyrosinase Antibody (C-term) (102-17294)immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Tyrosinase Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
 | Confocal immunofluorescent analysis of Tyrosinase Antibody (C-term)(Cat# 102-17294) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue). |
 | Tyrosinase Antibody (C-term) (Cat# 102-17294) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.
