Anti-U2AF1 (Center Y114) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q01081
Gene Id	7307
Gene Symbols	U2AF1
Protein Name / Synonyms	U2AF1 (Center Y114)
Clonality	Polyclonal
Preparation	This U2AF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 99-125 amino acids from the Central region of human U2AF1.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	FC, IHC-P, Western Blotting
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

U2AF1 belongs to the splicing factor SR family. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers.

Specificity

Predicted Reactivity

Mouse, Bovine

Other Names

U2AF1; U2AF35; U2AFBP; Splicing factor U2AF 35 kDa subunit; U2 auxiliary factor 35 kDa subunit; U2 small nuclear RNA auxiliary factor 1; U2 snRNP auxiliary factor small subunit

NCBI Accession #

NP_001020374.1;NP_001020375.1;NP_006749.1

Other Accession #

Q9D883; A1A4K8

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

27872

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50

	Western blot analysis of anti-U2AF1 Antibody (Center Y114) (Cat# 102-15150) in Ramos cell line lysates (35ug/lane).U2AF1(arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human lung carcinoma reacted with U2AF1 Antibody (Center Y114) (Cat# 102-15150), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	U2AF1 Antibody (Center Y114) (Cat# 102-15150) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Western blot analysis of U2AF1 (arrow) using rabbit polyclonal U2AF1 Antibody (Center Y114) (Cat# 102-15150). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the U2AF1 gene.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products