Anti-U2AF2 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P26368
Gene Id	11338
Gene Symbols	U2AF2
Protein Name / Synonyms	U2AF2, U2AF65, splicing factor U2AF 65 kDa subunit
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF, IP
Shipping Type	Blue ice
Storage	-20°C

Description

Background

U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:100
IP 1:50 - 1:200

Format

Calculated MW

53kDa

Observed MW

54kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 192-471 of human U2AF2 (NP_001012496.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using U2AF2 antibody (144-01936) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - U2AF2 Polyclonal Antibody (144-01936)

Immunofluorescence analysis of U2OS cells using U2AF2 antibody (144-01936). Blue: DAPI for nuclear staining.
Immunofluorescence - U2AF2 Polyclonal Antibody (144-01936)

Immunoprecipitation analysis of 200ug extracts of SW620 cells using 1ug U2AF2 antibody (144-01936). Western blot was performed from the immunoprecipitate using U2AF2 antibody (144-01936) at a dilition of 1:500.
Immunoprecipitation - U2AF2 Polyclonal Antibody (144-01936)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products