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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P63279 |
| Gene Id | 7329 |
| Gene Symbols | UBE2I |
| Protein Name / Synonyms | UBC9 (UBE2I) (N-term) |
| Clonality | Polyclonal |
| Preparation | This UBC9 (UBE2I) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human UBC9 (UBE2I). |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
UBE2I (Ubc9) is a member of the E2 family and is specific for the conjugation of SUMO to a variety of target proteins. SUMO conjugation to target proteins is mediated by a different, but analogous, pathway to ubiquitinylation. This E2 is unusual in that it interacts directly with protein substrates that are modified by sumoylation, and may play a role in substrate recognition. UBE2I can mediate the conjugation of SUMO-1 to a variety of proteins including RanGAP1 and PML without the requirement of an E3 ligase. UBE2I is essential for nuclear architecture and chromosome segregation.
Specificity
Predicted Reactivity
Mouse, Rat, Zebrafish, Chicken, Xenopus
Other Names
UBE2I; UBC9; UBCE9; SUMO-conjugating enzyme UBC9; SUMO-protein ligase; Ubiquitin carrier protein 9; Ubiquitin carrier protein I; Ubiquitin-conjugating enzyme E2 I; Ubiquitin-protein ligase I; p18
NCBI Accession #
NP_003336.1;NP_919235.1;NP_919236.1;NP_919237.1
Other Accession #
P63282; P63281; P63280; P63283; Q9DDJ0; Q9W6H5
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
18007
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100
 | Western blot analysis of anti-UBE2I Antibody (N-term) (Cat# 102-10731) in A2058 cell line lysates (35ug/lane). UBE2I(arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
 | Western blot analysis of UBE2I (arrow) using rabbit polyclonal UBE2I Antibody (S7) (Cat# 102-10731). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE2I gene. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.