Loading...
Loading...
Loading...
Loading...
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 7320 |
| Protein Name / Synonyms | UBE2B |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
E2-17kDa antibody, HHR6B antibody, HR6B antibody, RAD6B antibody, UBC2 antibody, UBE2B antibody, ubiquitin-protein ligase B antibody, ubiquitin carrier protein B antibody, ubiquitin-conjugating enzyme E2-17 kDa antibody, ubiquitin-conjugating enzyme E2 B antibody, RAD6 homolog B antibody, ubiquitin-conjugating enzyme E2B (RAD6 homolog) antibody, E2 protein antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
1XPBS, 1%BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
17 kDa
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 182 of UBE2B
Applications
Applications Tested
ICC/IF,Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of paraformaldehyde-fixed A431, using UBE2B(107-10223) antibody at 1:500 dilution.
UBE2B antibody detects UBE2B protein by western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg whole cell lysate/extract of 3xFlag-human UBE2B-transfected 293T cells
12 % SDS-PAGE
UBE2B antibody (107-10223) dilution: 1:5000
A. 30 µg 293T whole cell lysate/extract
B. 30 µg whole cell lysate/extract of 3xFlag-human UBE2B-transfected 293T cells
12 % SDS-PAGE
UBE2B antibody (107-10223) dilution: 1:5000
Sample (30 ug of whole cell lysate)
A: NIH-3T3 12% SDS PAGE
107-10223 diluted at 1:1000
A: NIH-3T3 12% SDS PAGE
107-10223 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.