Anti-UBE2D1 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P51668
Gene Id	7321
Gene Symbols	UBE2D1
Protein Name / Synonyms	UBE2D1 (C-term)
Clonality	Polyclonal
Preparation	This UBE2D1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-140 amino acids from the C-terminal region of human UBE2D1.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2D1 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases.

Specificity

Predicted Reactivity

Mouse, Rat, Bovine

Other Names

UBE2D1; SFT; UBC5A; UBCH5; UBCH5A; Ubiquitin-conjugating enzyme E2 D1; Stimulator of Fe transport; UBC4/5 homolog; UbcH5; Ubiquitin carrier protein D1; Ubiquitin-conjugating enzyme E2(17)KB 1; Ubiquitin-conjugating enzyme E2-17 kDa 1; Ubiquitin-protein ligase D1

NCBI Accession #

NP_003329.1

Other Accession #

D3ZDK2; P61080; Q2TA10; NP_003329

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

16602

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	Western blot analysis of lysates from Jurkat, Raji cell line (from left to right), using UBE2D1 Antibody (I126)(Cat# 102-14968). 102-14968 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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