UBE2L3 antibody, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 7332 |
| Protein Name / Synonyms | UBE2L3 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
E2-F1 antibody, L-UBC antibody, UBCH7 antibody, UbcM4 antibody, UBE2L3 antibody, ubiquitin-conjugating enzyme E2 L3 antibody, ubiquitin-protein ligase L3 antibody, ubiquitin carrier protein L3 antibody, ubiquitin-conjugating enzyme E2-F1 antibody, ubiquitin-conjugating enzyme UBCH7 antibody, ubiquitin-conjugating enzyme E2L 3 antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
18 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 92 and 154 of UBE2L3 (Uniprot ID# P68036)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using UBE2L3(107-10476) antibody at 1:100 dilution.
Immunoprecipitation of UBE2L3 protein from HeLa whole cell extracts using 5 µg of UBE2L3 antibody (107-10476).
Western blot analysis was performed using UBE2L3 antibody (107-10476).
EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Western blot analysis was performed using UBE2L3 antibody (107-10476).
EasyBlot anti-Rabbit IgG was used as a secondary reagent.
UBE2L3 antibody detects UBE2L3 protein by Western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 ug GL261 whole cell lysate/extract
C. 30 ug C8D30 whole cell lysate/extract
15 % SDS-PAGE
UBE2L3 antibody (107-10476) dilution: 1:1000
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 ug GL261 whole cell lysate/extract
C. 30 ug C8D30 whole cell lysate/extract
15 % SDS-PAGE
UBE2L3 antibody (107-10476) dilution: 1:1000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using UBE2L3(107-10476) antibody at 1:200 dilution.
Sample (30 ug of whole cell lysate)
A: Hela
B: Hep G2
12% SDS PAGE
107-10476 diluted at 1:1000
A: Hela
B: Hep G2
12% SDS PAGE
107-10476 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.