Anti-UBP1 (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q9NZI7
Gene Id	7342
Gene Symbols	UBP1
Protein Name / Synonyms	UBP1 (Center)
Clonality	Polyclonal
Preparation	This UBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 266-295 amino acids from the Central region of human UBP1.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	IHC-P, IF, Western Blotting
Research Area	Angiogenesis
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

UBP1 functions as a transcriptional activator in a promoter context-dependent manner. Modulates the placental expression of CYP11A1. Involved in regulation of the alpha-globin gene in erythroid cells. Activation of the alpha-globin promoter in erythroid cells is via synergistic interaction with TFCP2 (By similarity). Involved in regulation of the alpha-globin gene in erythroid cells. Binds strongly to sequences around the HIV-1 initiation site and weakly over the TATA-box. Represses HIV-1 transcription by inhibiting the binding of TFIID to the TATA-box.

Specificity

Predicted Reactivity

Mouse

Other Names

UBP1; LBP1; Upstream-binding protein 1; Transcription factor LBP-1

NCBI Accession #

NP_001121632.1;NP_001121633.1;NP_055332.3

Other Accession #

Q811S7; NP_001121632.1; NP_055332.3; NP_001121633.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

60491

Recommended Dilutions

IF: 1:10-50; WB: 1:1000; IHC: 1:10-50

	Fluorescent image of A549 cell stained with UBP1 Antibody (Center)(Cat# 102-12294).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with UBP1 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°).UBP1 immunoreactivity is localized to Nucleus significantly.
	UBP1 Antibody (Center) (Cat# 102-12294) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the UBP1 antibody detected the UBP1 protein (arrow).
	UBP1 Antibody (Center) (102-12294)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UBP1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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