Anti-UCH37 (UCHL5) (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q9Y5K5
Gene Id	51377
Gene Symbols	UCHL5
Protein Name / Synonyms	UCH37 (UCHL5) (N-term)
Clonality	Polyclonal
Preparation	This UCH37 (UCHL5) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-87 amino acids from the N-terminal region of human UCH37 (UCHL5).
Concentration (lot specific)	2.000 mg/ml
Recommended Applications	Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Covalent attachment of the C-terminus of ubiquitin to cellular proteins plays a role in a variety of cellular processes. Ubiquitin C-terminal hydrolysis is catalyzed by deubiquitinating (DUB) enzymes and is necessary for several functions, including liberation of monomeric ubiquitin from the precursors encoded by ubiquitin genes and recycling of ubiquitin monomers. There are 2 distinct families of DUBs, ubiquitin-specific proteases (UBPs) and ubiquitin C-terminal hydrolases (UCHs). Mayer and Wilkinson (1989) identified 4 distinct UCH activities from bovine thymus. All 4 were thiol proteases and had high-affinity binding sites for ubiquitin. Wilkinson et al. (1989) purified the predominant isozyme, UCHL3, and raised antibodies against it. By screening a human B-cell expression library with the antibodies, the authors isolated cDNAs encoding human UCHL3. Sequence comparisons revealed that the sequence of the predicted 230-amino acid human UCHL3 protein is 54% identical to that of UCHL1.

Specificity

Predicted Reactivity

Bovine, Pig

Other Names

UCHL5; UCH37; Ubiquitin carboxyl-terminal hydrolase isozyme L5; Ubiquitin C-terminal hydrolase UCH37; Ubiquitin thioesterase L5

NCBI Accession #

NP_001186190.1;NP_001186191.1;NP_001186192.1;NP_057068.1

Other Accession #

Q06AT3; Q9XSJ0

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

37607

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

The anti-UCHL5 Pab (Cat# 102-14983) is used in Western blot to detect UCHL5 in mouse kidney tissue lysate.

HE staining of frozen human ovarian cancer tissue reacted with the primary antibody at a 1:250 dilution. Levels using the antibody on frozen tissue array (A )correlated well with the mRNA expression levels detected by Agilent expression microarray (B). This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. 60X magnification. Data courtesy of Marlena Fejzo, University of California, Los Angeles.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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