Rabbit Anti-UCHL1 (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P09936 |
| Gene Id | 7345 |
| Gene Symbols | UCHL1 |
| Protein Name / Synonyms | UCHL1 (N-term) |
| Clonality | Polyclonal |
| Preparation | This UCHL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-46 amino acids from the N-terminal region of human UCHL1. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, IF, FC |
| Research Area | Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
UCHL1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons (Doran et al., 1983 [PubMed 6343558]).
Specificity
Predicted Reactivity
Bovine, Pig
Other Names
UCHL1; Ubiquitin carboxyl-terminal hydrolase isozyme L1; Neuron cytoplasmic protein 9.5; PGP 9.5; Ubiquitin thioesterase L1
NCBI Accession #
NP_004172.2
Other Accession #
Q6SEG5; P23356
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
24824
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50; IF: 1:10-50; FC: 1:10-50
 | Western blot analysis of UCHL1 (arrow) using rabbit polyclonal UCHL1-V31 (Cat# 102-14979). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the UCHL1 gene (Lane 2) (Origene Technologies). |
 | Western blot analysis of UCHL1-V31 (Cat# 102-14979) in CEM(lane 1), Jurkat(lane 2), Y79(lane 3) cell line and mouse brain tissue(lane 4) lysates (35ug/lane). UCHL1 (arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human brain tissue reacted with UCHL1 (Park5) antibody (N-term) (Cat# 102-14979), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | Confocal immunofluorescent analysis of UCHL1 Antibody (N-term)(Cat# 102-14979) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue). |
 | UCHL1 Antibody (N-term) (Cat# 102-14979) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.