Anti-UCHL3 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P15374
Gene Id	7347
Gene Symbols	UCHL3
Protein Name / Synonyms	UCHL3 (C-term)
Clonality	Polyclonal
Preparation	This UCHL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 195-225 amino acids from the C-terminal region of human UCHL3.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Covalent attachment of the C-terminus of ubiquitin to cellular proteins plays a role in a variety of cellular processes. Ubiquitin C-terminal hydrolysis is catalyzed by deubiquitinating (DUB) enzymes and is necessary for several functions, including liberation of monomeric ubiquitin from the precursors encoded by ubiquitin genes and recycling of ubiquitin monomers. There are 2 distinct families of DUBs, ubiquitin-specific proteases (UBPs) and ubiquitin C-terminal hydrolases (UCHs). Mayer and Wilkinson (1989) identified 4 distinct UCH activities from bovine thymus. All 4 were thiol proteases and had high-affinity binding sites for ubiquitin. Wilkinson et al. (1989) purified the predominant isozyme, UCHL3, and raised antibodies against it. By screening a human B-cell expression library with the antibodies, the authors isolated cDNAs encoding human UCHL3. Sequence comparisons revealed that the sequence of the predicted 230-amino acid human UCHL3 protein is 54% identical to that of UCHL1.

Specificity

Predicted Reactivity

Mouse, Rat, Bovine, Pig

Other Names

UCHL3; Ubiquitin carboxyl-terminal hydrolase isozyme L3; Ubiquitin thioesterase L3

NCBI Accession #

NP_001257881.1;NP_005993.1

Other Accession #

P58321; Q91Y78; Q06AB3; Q9JKB1; Q2TBG8

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

26183

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50

	Western blot analysis of lysates from 293, 293T, SW620, U-87 MG cell line (from left to right), using UCHL3 Antibody (C209) (Cat# 102-14982). 102-14982 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
	Western blot analysis of UCHL3 (Cat# 102-14982) in HepG2 cell line lysates (35ug/lane). UCHL3 (arrow) was detected using the purified Pab.
	Western blot analysis of his tag antibody in UCHL3 gene transfected 293 lysates (35ug/lane). His-tag UCHL3 protein (arrow) is detected.
	Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with UCHL3 Antibody (C-term)(Cat# 102-14982), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with UCHL3 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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