Anti-UFD1L Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Gene Id	7353
Protein Name / Synonyms	UFD1L
Concentration (lot specific)	1.02 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

UFD1 antibody, UFD1L antibody, UB fusion protein 1 antibody, ubiquitin fusion degradation protein 1 homolog antibody, ubiquitin fusion degradation 1 like (yeast) antibody

Specificity

Species Reactivity

Human, Mouse

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

35 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 307 of UFD1L (Uniprot ID# Q92890)

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

UFD1L antibody detects UFD1L protein by Western blot analysis.
A. 50 µg mouse lung lysate/extract
12 % SDS-PAGE
UFD1L antibody (107-10852) dilution: 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed A431, using UFD1L(107-10852) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded H441 xenograft, using UFD1L(107-10852) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)
A: A431
B: H1299
10% SDS PAGE107-10852 diluted at 1:10000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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