Anti-UGT1A1 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P22309
Gene Id	54658
Gene Symbols	UGT1A1
Protein Name / Synonyms	UGT1A1 (N-term)
Clonality	Polyclonal
Preparation	This UGT1A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 65-90 amino acids from the N-terminal region of human UGT1A1.
Concentration (lot specific)	0.420 mg/ml
Recommended Applications	Western Blotting, IF, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. The preferred substrate of this enzyme is bilirubin, although it also has moderate activity with simple phenols, flavones, and C18 steroids. Mutations in this gene result in Crigler-Najjar syndromes types I and II and in Gilbert syndrome.

Specificity

Other Names

UGT1A1; GNT1; UGT1; UDP-glucuronosyltransferase 1-1; Bilirubin-specific UDPGT isozyme 1; UDP-glucuronosyltransferase 1-A; UDP-glucuronosyltransferase 1A1

NCBI Accession #

NP_000454.1

Other Accession #

NP_000454.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

59591

Recommended Dilutions

WB: 1:1000; IF: 1:10-50; IHC: 1:10-50

	UGT1A1 Antibody (N-term) (Cat# 102-12232) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the UGT1A1 antibody detected the UGT1A1 protein (arrow).
	Confocal immunofluorescent analysis of UGT1A1 Antibody (N-term)(Cat# 102-12232) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
	UGT1A1 Antibody (N-term) (Cat# 102-12232)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UGT1A1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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