Anti-UHRF1 (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q96T88
Gene Id	29128
Gene Symbols	UHRF1
Protein Name / Synonyms	UHRF1 (Center)
Clonality	Polyclonal
Preparation	This UHRF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 229-257 amino acids from the Central region of human UHRF1.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, FC, IF
Research Area	Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

UHRF1 is a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone acetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint.

Specificity

Other Names

UHRF1; ICBP90; NP95; RNF106; E3 ubiquitin-protein ligase UHRF1; Inverted CCAAT box-binding protein of 90 kDa; Nuclear protein 95; Nuclear zinc finger protein Np95; RING finger protein 106; Transcription factor ICBP90; Ubiquitin-like PHD and RING finger domain-containing protein 1; Ubiquitin-like-containing PHD and RING finger domains protein 1

NCBI Accession #

NP_001041666.1;NP_037414.3

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

89814

Recommended Dilutions

WB: 1:1000; FC: 1:10-50; IF: 1:10-50

	UHRF1 Antibody (Center) (Cat# 102-18054) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the UHRF1 antibody detected the UHRF1 protein (arrow).
	UHRF1 Antibody (Center) (Cat# 102-18054) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Fluorescent confocal image of Hela cell stained with UHRF1 Antibody (Center)(Cat# 102-18054).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with UHRF1 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). UHRF1 immunoreactivity is localized to Nucleus significantly.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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