Synonyms
KINH antibody, KNS antibody, KNS1 antibody, UKHC antibody, KIF5B antibody, kinesin-1 heavy chain antibody, kinesin 1 (110-120kD) antibody, kinesin heavy chain antibody, ubiquitous kinesin heavy chain antibody, conventional kinesin heavy chain antibody, kinesin family member 5B antibody
Specificity
Format
Product Type
Primary antibody
Formulation
1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
110 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 901 and 963 of UKHC (Uniprot ID# P33176)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded Breast ca, using UKHC(107-10496) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: H1299
15% SDS PAGE
107-10496 diluted at 1:1500
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.