Anti-ULK2 (C-term) Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Gene Id	9706
Protein Name / Synonyms	ULK2, C-term
Concentration (lot specific)	1 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

ATG1B antibody, KIAA0623 antibody, Unc51.2 antibody, ULK2 antibody, serine/threonine-protein kinase ULK2 antibody, unc-51-like kinase 2 (C. elegans) antibody

Specificity

Species Reactivity

Human, Mouse

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

113 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 693 and 968 of ULK2 (Uniprot ID# Q8IYT8)

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using ULK2(107-10970) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)
A: NT2D1
B: PC-3
7.5% SDS PAGE
107-10970 diluted at 1:1000

Sample (30 ug of whole cell lysate)
A: NIH-3T3
7.5% SDS PAGE
107-10970 diluted at 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ULK2(107-10970) antibody at 1:200 dilution.

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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