Anti-ULK2 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q8IYT8
Gene Id	9706
Gene Symbols	ULK2
Protein Name / Synonyms	ULK2 (N-term)
Clonality	Polyclonal
Preparation	This ULK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 235-264 amino acids from the N-terminal region of human ULK2.
Concentration (lot specific)	2.000 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). Two human homologs of the yeast autophagy-specific kinase exist: ULK1(APG1) and ULK2. APG1 plays a critical role in regulating key elements of the autophagy pathway. APG1 stimulates autophagy, leading to autophagy-dependent restriction of cell growth and ultimately cell apoptosis at high levels of activity, and is a negative regulator of mTOR signaling.

Specificity

Predicted Reactivity

Mouse

Other Names

ULK2; KIAA0623; Serine/threonine-protein kinase ULK2; Unc-51-like kinase 2

NCBI Accession #

NP_001136082.1;NP_055498.3

Other Accession #

Q9QY01

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

112694

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50

	Western blot analysis of anti-ULK2 Antibody (N-term) (Cat# 102-17803) in K562 cell line lysates (35ug/lane). ULK2(arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human brain tissue reacted with ULK2 antibody (N-term) (Cat# 102-17803), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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