Anti-ULK3 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Mouse
Accession Number	Q6PHR2
Gene Id	25989
Gene Symbols	ULK3
Protein Name / Synonyms	Serine/threonine-protein kinase ULK3, Unc-51-like kinase 3, ULK3
Clonality	Monoclonal
Clone	1404CT148.89.25
Isotype	Kappa, IgG1
Recommended Applications	Western Blotting
Research Area	Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy.

Other Information

Format

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.

Calculated Molecular Weight (Da)

53444

Antigen Source

Human

Images

WB (1:1000)

Western blot analysis of lysates from Hela, LNCaP cell line, mouse testis and rat testis tissue lysates(from left to right), using ULK3 Antibody (C-term)(Cat. #102-20107). 102-20107 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35?g per lane.

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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