Anti-USF1 (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P22415
Gene Id	7391
Gene Symbols	USF1
Protein Name / Synonyms	USF1 (Center)
Clonality	Polyclonal
Preparation	This USF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 174-201 amino acids from the Central region of human USF1.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, FC, IF, IHC-P
Research Area	Cardiovascular Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

This gene encodes a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs. This gene has been linked to familial combined hyperlipidemia (FCHL). Two transcript variants encoding distinct isoforms have been identified for this gene.

Specificity

Predicted Reactivity

Bovine

Other Names

USF1; BHLHB11; USF; Upstream stimulatory factor 1; Class B basic helix-loop-helix protein 11; Major late transcription factor 1

NCBI Accession #

NP_001263302.1;NP_009053.1;NP_996888.1

Other Accession #

Q61069; Q6XBT4; NP_009053.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

33538

Recommended Dilutions

WB: 1:1000; FC: 1:10-50; IF: 1:10-50; IHC: 1:10-50

	USF1 Antibody (Center) (Cat# 102-10926) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the USF1 antibody detected the USF1 protein (arrow).
	USF1 Antibody (Center) (Cat# 102-10926) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Fluorescent confocal image of SK-BR-3 cell stained with USF1 Antibody (Center)(Cat# 102-10926). SK-BR-3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with USF1 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).USF1 immunoreactivity is localized to nucleus significantly.
	USF1 Antibody (Center) (Cat# 102-10926)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of USF1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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