Anti-USP5 Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Accession Number	P45974
Gene Id	8078
Gene Symbols	USP5
Protein Name / Synonyms	USP5
Clonality	Monoclonal
Preparation	This USP5 antibody is generated from a mouse immunized with a recombination protein from the human region of human USP5.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting
Research Area	Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys- 63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.

Specificity

Other Names

Ubiquitin carboxyl-terminal hydrolase 5, Deubiquitinating enzyme 5, Isopeptidase T, Ubiquitin thioesterase 5, Ubiquitin-specific-processing protease 5, USP5, ISOT

Format

Type

Purified Mouse Monoclonal Antibody (Mab)

Calculated Molecular Weight (Da)

95786

Recommended Dilutions

WB: 1:2000

Western blot analysis of lysates from U251, PC-3 cell line, mouse brain, rat brain and liver tissue lysates (from left to right), using USP5 Antibody(Cat# 102-10522). 102-10522 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products