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Anti-VAM1 (N-term) Antibody
Catalog #: 107-10725
VAM1 antibody [N1], N-Terminus, 1 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Gene Id | 51678 |
| Protein Name / Synonyms | VAM1, N-term |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
PALS2 antibody, VAM-1 antibody, VAM1 antibody, p55T antibody, MPP6 antibody, MAGUK p55 subfamily member 6 antibody, protein associated with Lin7 2 antibody, VELI-associated MAGUK 1 antibody, MAGUK protein p55T antibody, "membrane protein, palmitoylated 6 (MAGUK p55 subfamily member 6) antibody"
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
61 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 1 and 44 of VAM1 (Uniprot ID# Q9NZW5)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded Colon, using VAM1(107-10725) antibody at 1:100 dilution.
VAM1 antibody [N1], N-term detects VAM1 protein at on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
VAM1 antibody [N1], N-term (107-10725) dilution: 1:500.
Sample: Paraffin-embedded mouse intestine.
VAM1 antibody [N1], N-term (107-10725) dilution: 1:500.
Sample (50 ug of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
107-10725 diluted at 1:1000
A: mouse brain
7.5% SDS PAGE
107-10725 diluted at 1:1000
Sample (50 ug of whole cell lysate)
A: Rat brain
7.5% SDS PAGE
107-10725 diluted at 1:1000
A: Rat brain
7.5% SDS PAGE
107-10725 diluted at 1:1000
Sample (30 ug of whole cell lysate)
A: NT2D1
7.5% SDS PAGE
107-10725 diluted at 1:1000
A: NT2D1
7.5% SDS PAGE
107-10725 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.