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Anti-VASP Antibody
Catalog #: 144-61511
Phospho-VASP-S157 pAb
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P50552 |
| Gene Id | 7408 |
| Gene Symbols | VASP |
| Protein Name / Synonyms | VASP, vasodilator-stimulated phosphoprotein |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IP 1:50 - 1:100
IHC 1:50 - 1:100
IP 1:50 - 1:100
Format
Calculated MW
39kDa
Observed MW
50kDa
Immunogen
A synthetic phosphorylated peptide around S157 of human VASP (NP_003361.1).
Modification
Phospho S157
Storage
-20°C
Images
Western blot analysis of extracts of A431 cells,using Phospho-VASP-S157 antibody (144-61511) at 1:2000 dilution. A431 cells were treated by Forskolin (10uM) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 1s.
Immunoprecipitation analysis of 200ug extracts of A-431 cells, using 3 ug Phospho-VASP-S157 pAb (144-61511). Western blot was performed from the immunoprecipitate using Phospho-VASP-S157 pAb (144-61511) at a dilition of 1:1000. A-431 cells were treated by Forskolin (10 uM) at 37? for 30 minutes after serum-starvation overnight.
Expiration:
12 months from the date of shipment when stored properly.