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Anti-Vaspin Antibody, Biotinylated
Catalog #: RB-08-0024B-50
Rabbit anti-Vaspin Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | NM_173850 |
| Clonality | Polyclonal |
| Immunogen | Immunogen was synthetic peptide derived from Human Vaspin. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbitt serum by ammonium sulphate precipitation followed by Protein A/G affinity chromatography. |
| Isotype | IgG |
| Recommended Applications | ELISA (recommended work dilution= 1- 0.1 µg/ml (at least)), Western Blotting (recommended work dilution= 1:1000-2000 (at least)), Enzyme Immunoassay (EIA)(recommended work dilution= Sensitivity (55.53 pM)) |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Visceral adipose tissue-derived serpin (Vaspin), a member of serine protease inhibitor family, is a visceral adipose tissue-derived adipokine with potential antiprotease properties. Vaspin cDNA was isolated by from visceral white adipose tissues (WATs) of Otsuka Long-Evans Tokushima fatty (OLETF) rat, an animal model of abdominal obesity with type 2 diabetes. Human, mouse and rat Vaspin is made up of 395, 394, and 392 amino acids respectively. Vaspin belongs to serine protease inhibitor family and it also shows about 40% homology with 1-antitrypsin. Vaspin ameliorates certain aberrations seen in the diabetic obesity metabolic syndrome by sensitizing insulin action, especially in WATs. Research on Vaspin has been trying for the identification of the potential protease substrate leading to the development of antiprotease inhibitor therapy, which could facilitate the improvement of insulin sensitivity in this metabolic syndrome.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
The rabbit anti-human Vaspin antibody detects specifically target derived from human recombinant protein as well as cell lysates at various concentrations. Cross reactivity with mouse and rat are expected from sequence similarity.
Related Products
Vaspin antibody(cat# RB-08-0002) ; Vaspin Immunoassay Kit (EIA, cat# EIA-VAP-1) ; Obesity antibody arrays(cat#AAH-OBS-1-1)
References
- Hida K, et.al.,(2005). Visceral adipose tissue-derived serine protease inhibitor: a unique insulinsensitizing adipocytokine in obesity. Proc Natl Acad Sci USA. 102(30):10610-5.
- Wada J (2008). Vaspin: a novel serpin with insulin-sensitizing effects. Expert Opin Investig Drugs 17(3):327-33.
Expiration:
12 months from the date of shipment when stored properly.