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Anti-Vaspin Antibody
Catalog #: RB-08-0024
Rabbit Anti-Human Vaspin Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | NM_173850 |
| Clonality | Polyclonal |
| Immunogen | Immunogen was synthetic peptide derived from Human Vaspin. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbitt serum by ammonium sulphate precipitation followed by Protein A/G affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution= 1- 0.1?g/ml (at least)), Western Blotting (recommended work dilution= 1:1000-2000 (at least)), Enzyme Immunoassay (EIA)(recommended work dilution= Sensitivity (55.53 pM)) |
| Reconstitution | Supplied as lyophilized purified antibody. The antibody is stable for at least one year from the data of receipt when stored at -20°C to -70°C. Reconstituted antibody (recommended with H20 1 mg/ml) can also be aliquoted and stored frozen at -20°C to -70°C in a manual defrost freezer for months without detectable loss activity. Upon reconstitution, the antibody can be stored at 4°C for 1 month. If 1 ml of H20 is used, the antibody concentration will be 100 ?g/ml. Please avoid freeze-thaw cycles. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Visceral adipose tissue-derived serpin (Vaspin), a member of serine protease inhibitor family, is a visceral adipose tissue-derived adipokine with potential antiprotease properties. Vaspin cDNA was isolated by from visceral white adipose tissues (WATs) of Otsuka Long-Evans Tokushima fatty (OLETF) rat, an animal model of abdominal obesity with type 2 diabetes. Human, mouse and rat Vaspin is made up of 395, 394, and 392 amino acids respectively. Vaspin belongs to serine protease inhibitor family and it also shows about 40% homology with 1-antitrypsin. Vaspin ameliorates certain aberrations seen in the diabetic obesity metabolic syndrome by sensitizing insulin action, especially in WATs. Research on Vaspin has been trying for the identification of the potential protease substrate leading to the development of antiprotease inhibitor therapy, which could facilitate the improvement of insulin sensitivity in this metabolic syndrome.
Specificity
The rabbit anti-human Vaspin antibody detects specifically target derived from human recombinant protein as well as cell lysates at various concentrations. Cross reactivity with mouse and rat are expected from sequence similarity.
Storage
Store at -20°C, if not intended for use within a month, Keep it at 4°C and minimize freezing and thawing when use.
Related Products
Vaspin antibody(cat# RB-08-0002) ; Vaspin Immunoassay Kit (EIA, cat# EIA-VAP-1) ; Obesity antibody arrays(cat#AAH-OBS-1-1)
References
- Hida K, et.al.,(2005). Visceral adipose tissue-derived serine protease inhibitor: a unique insulinsensitizing adipocytokine in obesity. Proc Natl Acad Sci USA. 102(30):10610-5.
- Wada J (2008). Vaspin: a novel serpin with insulin-sensitizing effects. Expert Opin Investig Drugs 17(3):327-33.
Expiration:
12 months from the date of shipment when stored properly.