Rabbit Anti-Human VCP (C-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P55072 |
| Gene Id | 7415 |
| Gene Symbols | VCP |
| Protein Name / Synonyms | VCP (C-term) |
| Clonality | Polyclonal |
| Preparation | This VCP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 726-755 amino acids from the C-terminal region of human VCP. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC |
| Research Area | Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
VCP is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation.
Specificity
Predicted Reactivity
Mouse, Rat, Zebrafish, Bovine, Pig, Xenopus
Other Names
VCP; Transitional endoplasmic reticulum ATPase; 15S Mg(2+)-ATPase p97 subunit; Valosin-containing protein
NCBI Accession #
NP_009057.1
Other Accession #
P23787; P46462; P03974; Q01853; Q7ZU99; Q3ZBT1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
89322
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50
 | Western blot analysis of VCP Antibody (C-term) (Cat# 102-17016) in Jurkat, 293, MDA-MB231 cell line lysates (35ug/lane). VCP (arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human brain tissue reacted with VCP Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | Flow cytometric analysis of MDA-231 cells using VCP Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.