Anti-VCP (N-term) Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Gene Id	7415
Protein Name / Synonyms	VCP, N-term
Concentration (lot specific)	1 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

IBMPFD antibody, MGC131997 antibody, MGC148092 antibody, MGC8560 antibody, TERA antibody, p97 antibody, VCP antibody, yeast Cdc48p homolog antibody, 15S Mg(2+)-ATPase p97 subunit antibody, TER ATPase antibody, transitional endoplasmic reticulum ATPase antibody, valosin-containing protein antibody, valosin containing protein antibody

Specificity

Species Reactivity

Human, Rat, Mouse

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

89 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 206 of VCP (Uniprot ID# P55072)

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Sample (50 ug of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
107-11045 diluted at 1:5000

VCP antibody detects VCP protein by Western blot analysis.
A. 50 µg rat brain lysate/extract
7.5 % SDS-PAGE
VCP antibody (107-11045) dilution: 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using VCP(p97)(107-11045) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using VCP(p97)(107-11045) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)
A: Hela
B: Hep G2
C: Molt-4
D: Raji
7.5% SDS PAGE
107-11045 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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