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ISO 13485:2016 cGMP
Anti-VEGF / VEGF-A Antibody, Biotinylated
Catalog #: DS-PB-02109
Goat Anti-Human VEGF / VEGF-A [+Biotin]
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Specificity | This product specifically recognizes human VEGF (vascular endothelial growth factor), a heparin-binding cytokine and member of the PDGF/VEGF growth factor family, which exists as several splice variants of the VEGF gene. VEGF, originally named vascular permeability factor, due to its ability to act as vasodilator and increase microvascular permeability, is a potent stimulator of angiogenesis of both normal and cancerous cells, and acts as a regulator of vasculogenesis. The functional forms of VEGF exist as disulphide-linked homodimers and are classified according to the number of amino acid residues within a constitutive polypeptide chain e.g VEGF165. All VEGF family members act through binding to the tyrosine kinase receptors VEGFR-1 (Flt-1) and VEGFR-2 (KDR/CD309), whilst the closely-related VEGF-C and VEGF-D, can also bind to VEGFR-3 (Flt-4). |
| Host Species | Goat |
| Protein Name / Synonyms | VEGF / VEGF-A |
| Preparation | Antisera to human VEGF were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography. |
| Concentration (lot specific) | IgG concentration 0.1 mg/ml after reconstitution. |
| Recommended Applications | ELISA (dilution: 0.1, 0.2ug/ml), Western Blotting |
| Reconstitution | Reconstitute with 0.5ml sterile PBS containing 0.1% Bovine Serum Albumin.. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution. For long term storage, the addition of 0.09% sodium azide is recommended. |
| Research Area | Angiogenesis, Cardiovascular Disease, Vascular Biology |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
This product may be used under either reducing or non-reducing western blotting conditions.
Storage/Stability
Prior to reconstitution store at +4 °C. Following reconstitution store at -20 °C.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.
Format
Supplied as:
Purified IgG conjugated to Biotin - lyophilized
Formulation:
Phosphate buffered saline
Reconstitution:
Reconstitute with 0.5ml sterile PBS containing 0.1% Bovine Serum Albumin.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution. For long term storage, the addition of 0.09% sodium azide is recommended.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution. For long term storage, the addition of 0.09% sodium azide is recommended.
Preparation
Antisera to human VEGF were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Immunogen:
Recombinant human VEGF
Preservative:
None present
Expiration:
12 months from the date of shipment when stored properly.