Anti-VEGF / VEGF-A Antibody

Catalog #: DS-PB-02328

Goat Anti-Human VEGF / VEGF-A

Product Description

Specifications

Size	0.1 mg
Estimated Lead Time	1-2 weeks
Species	Human
Specificity	This product specifically recognizes human VEGF (vascular endothelial growth factor), a heparin-binding cytokine and member of the PDGF/VEGF growth factor family, which exists as several splice variants of the VEGF gene. VEGF, originally named vascular permeability factor due to its ability to act as vasodilator and increase microvascular permeability, is a potent stimulator of angiogenesis of both normal and cancerous cells, and acts as a regulator of vasculogenesis. The functional forms of VEGF exist as disulphide-linked homodimers and are classified according to the number of amino acid residues within a constitutive polypeptide chain e.g VEGF₁₆₅. All VEGF family members act through binding to the tyrosine kinase receptors VEGFR-1 (Flt-1) and VEGFR-2 (KDR/CD309), whilst the closely-related VEGF-C and VEGF-D, can also bind to VEGFR-3 (Flt-4).
Host Species	Goat
Protein Name / Synonyms	VEGF / VEGF-A
Preparation	Antisera to human VEGF were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Concentration (lot specific)	IgG concentration 1.0 mg/ml after reconstitution.
Recommended Applications	ELISA (dilution: 0.1, 0.2ug/ml), Functional Assays, Western Blotting
Reconstitution	Reconstitute with 0.1ml distilled water. For long term storage, the addition of 0.09% soidum azide is recommended. NB: For functional studies do not add azide.
Research Area	Angiogenesis, Cardiovascular Disease, Vascular Biology
Shipping Type	Ambient temperature
Storage	-20°C

Description

This product may be used under either reducing or non-reducing western blotting conditions.

Storage/Stability

Prior to reconstitution store at +4 °C. Following reconstitution store at -20 °C.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.

Format

Supplied as:

Purified IgG - lyophilized

Formulation:

Phosphate buffered saline

Reconstitution:

Reconstitute with 0.1ml distilled water. For long term storage, the addition of 0.09% soidum azide is recommended. NB: For functional studies do not add azide.

Format Type:

Purified

Preparation

Antisera to human VEGF were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.

Immunogen:

Recombinant human VEGF

Preservative:

None present

Expiration:

12 months from the date of shipment when stored properly.

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