Anti-VEGFB (VEGF2) (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P49765
Gene Id	7423
Gene Symbols	VEGFB
Protein Name / Synonyms	VEGFB (VEGF2) (Center)
Clonality	Polyclonal
Preparation	This VEGFB (VEGF2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 110-139 amino acids from the Central region of human VEGFB (VEGF2).
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	Angiogenesis, Vascular Biology
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Vascular endothelial growth factors (VEGFs) are a family of closely related growth factors having a conserved pattern of eight cysteine residues and sharing common VEGF receptors. VEGFs stimulate endothelial cells, induce angiogenesis, promote cell migration, increase vascular permeability, and inhibit apoptosis. VEGFB has structural similarities to VEGF and PIGF and is frequently co-expressed with VEGF. There are two alternatively spliced isoforms of VEGFB: VEGFB 167 and VEGFB 186. VEGFB 167, a highly basic heparin-binding protein, remains with the cell or extracellular matrix whereas, VEGFB 186 is readily secreted. VEGFB stimulates endothelial cell proliferation. VEGFB binds to the tyrosine kinase receptor VEGFR1 (flt1) and does not bind to VEGFR2. Vascular Endothelial Growth Factor B is widely expressed but is most abundant in heart, skeletal muscle, and pancreas. It has been suggested that VEGFB expression in human primary breast cancers is associated with lymph node metastasis. The genes that encode VEGFB have been mapped to chromosome 11q13.

Specificity

Other Names

VEGFB; VRF; Vascular endothelial growth factor B; VEGF-related factor

NCBI Accession #

NP_001230662.1;NP_003368.1

Other Accession #

Q16528

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

21602

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50

	Western blot analysis of VEGF2 (arrow) using rabbit polyclonal VEGF2 Antibody (Center)(Cat# 102-16731). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the VEGF2 gene (Lane 2) (Origene Technologies).
	Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with VEGF2 antibody (Center)(Cat# 102-16731), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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