Anti-VEGFR2 (FLK1/KDR) (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P35968
Gene Id	3791
Gene Symbols	KDR
Protein Name / Synonyms	VEGFR2 (FLK1/KDR) (C-term)
Clonality	Polyclonal
Preparation	This VEGFR2 (FLK1/KDR) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1326-1356 amino acids from the C-terminal region of human VEGFR2 (FLK1/KDR).
Concentration (lot specific)	1.270 mg/ml
Recommended Applications	Western Blotting, IF, FC, IHC-P
Research Area	Angiogenesis, Cancer, Autophagy, Vascular Biology
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

KDR (FLK1) is a receptor for VEGF or VEGFC. This protein has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability.

Specificity

Other Names

KDR; FLK1; VEGFR2; Vascular endothelial growth factor receptor 2; Fetal liver kinase 1; Kinase insert domain receptor; Protein-tyrosine kinase receptor flk-1; CD_antigen=CD309; Flags: Precursor

NCBI Accession #

NP_002244.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

151527

Recommended Dilutions

WB: 1:1000; IF: 1:10-50; FC: 1:10-50; IHC: 1:50-100

	Western blot analysis of anti-KDR/FLK1 Pab (Cat# 102-17506) in HeLa cell lysate. VEGFR2 (KDR) (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
	Confocal immunofluorescent analysis of VEGFR2 (FLK1/KDR) Antibody (C-term)(Cat# 102-17506) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
	VEGFR2 (FLK1/KDR) Antibody (C-term) (Cat# 102-17506) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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