Anti-Vimentin (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P08670
Gene Id	7431
Gene Symbols	VIM
Protein Name / Synonyms	Vimentin (C-term)
Clonality	Polyclonal
Preparation	This Vimentin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 430-457 amino acids from the C-terminal region of human Vimentin.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.

Specificity

Predicted Reactivity

Mouse, Rat, Bovine, Chicken, Hamster, Pig, Monkey, Xenopus

Other Names

VIM; Vimentin

NCBI Accession #

NP_003371.2

Other Accession #

P31000; P02543; P20152; Q4R4X4; P48670; P09654; P48616; P24790

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

53652

Recommended Dilutions

IF: 1:100; WB: 1:1000; IHC: 1:10-50; FC: 1:10-50

	Fluorescent confocal image of SY5Y cells stained with Vimentin (C-term) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with 102-15110 Vimentin (C-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). Note the highly specific localization of the Vimentin immunosignal to the cytoskeleton, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000026025).
	Western blot analysis of Vimentin antibody (C-term) (Cat# 102-15110) in NCI-H460 cell line lysates (35ug/lane). Vimentin (arrow) was detected using the purified Pab .
	Vimentin Antibody (C-term) (Cat# 102-15110)immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Vimentin Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
	Vimentin Antibody (C-term) (Cat# 102-15110) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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