VPS11 antibody, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 55823 |
| Protein Name / Synonyms | VPS11 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
END1 antibody, PEP5 antibody, RNF108 antibody, hVPS11 antibody, VPS11 antibody, RING finger protein 108 antibody, vacuolar protein sorting-associated protein 11 homolog antibody, vacuolar protein sorting protein 11 antibody, vacuolar protein sorting 11 homolog (S. cerevisiae) antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
108 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 300 and 392 of VPS11 (Uniprot ID# Q9H270)
Applications
Applications Tested
ICC/IF,Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
VPS11 antibody detects VPS11 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in -20? 100% MeOH for 5 min.
Green: VPS11 protein stained by VPS11 antibody (107-10700) diluted at 1:500.
Blue: Hoechst 33343 staining.
Sample: HeLa cells were fixed in -20? 100% MeOH for 5 min.
Green: VPS11 protein stained by VPS11 antibody (107-10700) diluted at 1:500.
Blue: Hoechst 33343 staining.
VPS11 antibody detects VPS11 protein at cytosol on Ca922 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Ca922 xenograft.
VPS11 antibody (107-10700) dilution: 1:500.
Sample: Paraffin-embedded Ca922 xenograft.
VPS11 antibody (107-10700) dilution: 1:500.
Sample(30 µg of whole cell lysate)
A:293T
B:Hep G2
7.5% SDS PAGE
107-10700 diluted at 1:500
A:293T
B:Hep G2
7.5% SDS PAGE
107-10700 diluted at 1:500
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.