Anti-VTA1 Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Gene Id	51534
Protein Name / Synonyms	VTA1
Concentration (lot specific)	0.74 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

C6orf55 antibody, DRG-1 antibody, DRG1 antibody, FLJ27228 antibody, LIP5 antibody, My012 antibody, SBP1 antibody, VTA1 antibody, dopamine-responsive gene 1 protein antibody, homolog of mouse SKD1-binding protein 1 antibody, SKD1-binding protein 1 antibody, vacuolar protein sorting-associated protein VTA1 homolog antibody, LYST-interacting protein 5 antibody, Vps20-associated 1 homolog (S. cerevisiae) antibody

Specificity

Species Reactivity

Human

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

34 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 276 of VTA1 (Uniprot ID# Q9NP79)

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using VTA1(107-10678) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:500.

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using VTA1(107-10678) antibody at 1:500 dilution.

Sample(30 ug whole cell lysate)
A:H1299
B:HeLa S3
C:MOLT4
12% SDS PAGE
107-10678 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

Featured Products