Anti-VTN Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P04004
Gene Id	7448
Gene Symbols	VTN
Protein Name / Synonyms	VTN, V75, VN, VNT, vitronectin
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is a member of the pexin family. It is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. It is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW

54kDa

Observed MW

65kDa, 75kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 364-478 of human VTN (NP_000629.3).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using VTN antibody (144-61313) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 20s.
Western blot - VTN Polyclonal Antibody (144-61313)

Immunohistochemistry of paraffin-embedded mouse lung using VTN antibody (144-61313) at dilution of 1:100 (40x lens).
Immunohistochemistry - VTN Polyclonal Antibody (144-61313)

Immunohistochemistry of paraffin-embedded mouse leydig cells using VTN antibody (144-61313) at dilution of 1:100 (40x lens).
Immunohistochemistry - VTN Polyclonal Antibody (144-61313)

Expiration:

12 months from the date of shipment when stored properly.

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